Parameters | Expression Parameters | Apply data from autosomes for normalization

Apply data from autosomes for normalization
Check this option to separate observations from non-autosomes (X and Y chromosomes) from those for the autosomes prior to normalization.
This separation must be done when there are different numbers of males and females in the study to prevent skewing of the data resulting from different numbers of non-autosomes.
The observations from the autosomes are normalized independently.
For each observation from non-autosomes, normalized data from the nearest smaller and nearest larger autosomes are interpolated.
Note: Checking this box deactivates the parameters on the Reference Set tab.