Processes | Expression Parameters | Apply data from autosomes for normalization

Apply data from autosomes for normalization
Check this option to separate observations from non-autosomes (X and Y chromosomes ) from those for the autosomes prior to normalization .
This separation must be done when there are different numbers of males and females in the study to prevent skewing of the data resulting from different numbers of non-autosomes.
The observations from the autosomes are normalized independently.
For each observation from non-autosomes, normalized data from the nearest smaller and nearest larger autosomes are interpolated.
Note : Checking this box deactivates the parameters on the Reference Set tab.