Speeding up assay development with automated workflow for assessing plate bias of microtiter assays

Speeding Up Assay Development

Tomasz Witkos
Associate Director

Hadley Myers
System Engineer Manager for N/W EMEA

Understanding positional effects in microtiter plate-based assays is an important step in developing robust potency assays for testing biologics. Here, you'll see a bespoke JMP application that automates the analysis of multiple-plate experiments aimed at detecting plate bias. The application, using the raw output plate reader file, creates plate map visualizations, calculates IC50/EC50 of drug dose-response curves, and analyses row and column variabilities across plates. What typically took scientists half a day of analysis, error-prone manual formatting of datasets, and the need for external software for curve fitting, can now be achieved in JMP in under one minute.